Our Tyrosine Kinase profiling are produced using a cell-based platform first developed by Mathey-Prevot and colleagues. It relies on constitutively activated kinases providing cytokine-independent growth to IL3-independent Ba/F3 (Pro-B) and FDC-P1 (Myeloid) cell lines. Upon addition of inhibitory compounds blocking the kinase function, the lines undergo cell death, resulting in a lower measurement of residual ATP (a surrogate marker for viability).
We offer two basic profiling packages; dose ranging evaluates three different concentrations arrayed as two full-log serial dilutions of your stock solution, while dose profiling evaluates ten concentrations arrayed as nine half-log serial dilutions. All evaluations are performed in duplicate and include parallel evaluation of parental control cells and dose-profiling of an internal reference standard against the target assay, both at no charge.
Want to learn more?
See our KinoScope and KinoCore for more information on our proprietary spatial ligand-binding mapping software and evaluations of compound activities across the tyrosine Kinome.