Compound Submission and Processing

The procedures specified below are observed for all customer-provided compounds. Deviation(s) from this protocol are permitted but will incur a processing surcharge commensurate with the complexity/operational burden of the requested deviation(s).

All compounds handled by ACD are to be provided in one of two forms:

  1. A pre-weighed solid.
  2. Solvated in DMSO.

When processing a new arrival, the compound will be stored at the same temperature at which it was shipped – unless the customer specifies an alternate storage condition. When launching the study, compounds are processed as follows:

  • Contents are warmed to room temperature.
  • If in solid form, the compound is resuspended in a fresh aliquot of anhydrous DMSO to a working concentration not to exceed 10 mM (the customer may specify a lower starting concentration in the application).
  • The solvated compound is examined visually and appearance documented with respect to color and clarity.
    • If cloudy, the solution is vortexed three times for 30 seconds each and visualized.
    • If the solution remains turbid or particulate, the solution is heated in a 37C water bath for ten minutes and again vortexed three times.
    • Any residual particulate matter is eliminated as follows:
      • The solution is transferred to a clean 1.5 mL polypropylene microfuge tube.
      • The solution is spun for five minutes at 14,000 x g.
      • The supernatant is collected and transferred to  a clean 1.5 mL polypropylene microfuge tube.
  •  The condition of each processed compound is photo-documented (pre- and post-processing when necessary) and appropriate serial dilutions prepared.
  • Unused material is retained in its original vial (unless cleared of particulates) and returned to the same storage condition specified by the customer.

Once compound plate preparation is complete, diluted compounds are stamped onto the assay plate using a V & P Scientific pin tool apparatus. When finished, the compound plate is sealed with foil and stored at room temperature protected from light. Should one or more assays perform outside of specified parameters, the foil seal is removed and the experiment is repeated as necessary to complete the data set. Once complete, the compound plate is again sealed with a new application of foil and returned to room temperature storage. This process is repeated as necessary until the study has been completed.

All customer compound plates are retained at RT for a period of four weeks and logged for destruction when the retention period expires. At expiration, the foil seal is removed and the plate placed in a fume hood until all liquid has evaporated. It is then discarded per our institutional EHS-specified protocol.

Unused material is logged and stored for a period of six months using the same storage conditions specified by the customer, after which it is destroyed following the same procedures specified above for compound plates.